Botanical from Piper capense Fruit Can Help to Combat the Melanoma as Demonstrated by In Vitro and In Vivo Studies

Figure 3

PCFE treatment modulates the expression of EMT markers in vitro. PCFE alone and in combination with dacarbazine upregulates the expression of FITC-tagged (green) E-cadherin and downregulates PE-tagged (red) vimentin of B16-F10 cells as compared to the untreated control (a). Western blot analysis and densitometric plot also showed significant upregulation of E-cadherin and downregulation of vimentin as well as CD133 (b, c). Statistical significance of each treatment group with untreated control is analyzed by one-way ANOVA test (_ANOVA < 0.0001) followed by post hoc Tukey’s test. Data are represented as mean ± SD of triplicate determinations from their independent experiments; ns: not significant, value <0.05, value <0.01, and value <0.0001 versus untreated control and among each group.

(a)

(b)

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