Huiyang Shengji Extract Improve Chronic Nonhealing Cutaneous through the TGF-β1/Smad3 Signaling Pathway

Table 1

Overview of primers used for qRT-PCR analysis.

Target gene	Direction	Sequence (5′-3′)	Amplicon size (bp)
CCL2	Forward	ATAGGAAGATCTCAGTGCAGAGGCTC	175
	Reverse	TTCGGAGTTTGGGTTTGCTTGT
CCL7	Forward	TCAATAAGAAAATCCCTAAGCAGAG	142
	Reverse	AAAGTCCTGGACCCACTTCTGT
IL-6	Forward	AAAGATGGCTGAAAAAGATGGATG	88
	Reverse	CAAACTCCAAAAGACCAGTGATGAT
MMP-1	Forward	ACGATTCGGGGAGAAGTGAT	120
	Reverse	AAGCCCATTTGGCAGTTGTG
MMP-2	Forward	GAATGCCATCCCCGATAACCT	116
	Reverse	TTCACGCTCTTCAGACTTTGGTTC
MMP-3	Forward	CTGAAGACTTTCCAGGGATTGACT	118
	Reverse	TGTCACTTTCTTTGCATTTGGGT
MMP-4	Forward	GGTGCTGAAGGCAGGGACTC	136
	Reverse	CAGTGGCATCTGAGGGAAAGG
TIMP-1	Forward	TACACTGTTGGCTGTGAGGAATG	140
	Reverse	CAAGGTGACGGGACTGGAAG
C0L1A1	Forward	ACAAGAGGCATGTCTGGTTCG	237
	Reverse	CGGATCTCGATCTCGTTGGA
COL3A1	Forward	CTACACAGTTCTGGAGGATGGTTGC	203
	Reverse	TTTTGTTGGGATTTCAGATAGAGTTT
18srRNA	Forward	GTAACCCGTTGAACCCCATT	151
	Reverse	CCATCCAATCGGTAGTAGCG