Anticholinesterase Activity of Eight Medicinal Plant Species: In Vitro and In Silico Studies in the Search for Therapeutic Agents against Alzheimer’s Disease

Table 2

Anticholinesterase inhibition (IC₅₀, µg/mL) of eight plant extracts and galantamine (positive control).

Species name	AChE inhibition assay	BChE inhibition assay
	IC50 (µg/mL)	IC50 (µg/mL)
Blumea lacera (Burm.f.) DC.	150 ± 11^a	297 ± 13^c
Byttneria pilosa Roxb.	221 ± 2^a,b	536 ± 23^d
Clerodendrum infortunatum L.	598 ± 50^d	971 ± 77^e
Cyclea barbata Miers	176 ± 14^a	124 ± 2^a
Mikania micrantha Kunth	314 ± 15^b	216 ± 25^b
Smilax guianensis Vitman	205 ± 31^a	>1000^f
Spermacoce articularis L.f.	460 ± 126^c	>1000^f
Thunbergia grandiflora Roxb.	252 ± 22^a,b	576 ± 64^d
Galantamine	0.92 ± 0.02 (2.49 ± 0.05 µM)	5.97 ± 0.97 (16.2 ± 2.6 µM)

Values are expressed as mean ± SD (n = 3). Different letters indicate statistically significant differences (p < 0.05, Tukey test). IC50 represents the half-maximal inhibitory concentration; AChE, acetylcholinesterase; BChE, butyrylcholinesterase.