Research Article

Bacterially Converted Oat Active Ingredients Enhances Antioxidative and Anti-UVB Photoaging Activities

Table 1

LC/MS analysis conditions.

ItemConditions

ColumnBEH 2.1 (2.7 mm × 50 mm, 1.8 μm)
Column TeM40

Mobile phaseA: 0.1% folic acid (FA)
B: Acetonitrile (ACN)

Flow rate0.5 mL/min
Injection volume3 μL

GradientMinAB
0955
0.5955
15050
1.55050
2595
3595
5955

ESI + capillary2KV
Source TeM desolvation TeM250
Source gas flow (L/Hr)600
Source temp150

Avenanthramide A: ES+, chan reaction: 300.18 > 147.11, cone voltage: 32, col. energy: 10, avenanthramide B: ES+, chan reaction: 316.30 > 163.11, cone voltage: 22, col. energy: 10, avenanthramide C: ES+, chan reaction: 330.19 > 177.12, cone voltage: 42, col. energy: 12, p-coumaric acid: ES, chan reaction: 163.10 > 119.10, cone voltage: 40, col. energy: 13, caffeic acid: ES, chan reaction: 179.10 > 135.10, cone voltage: 35, col. energy: 15, ferulic acid: ES, chan reaction: 193.10 > 134.10, cone voltage: 40, col. energy: 15.