Research Article
Protective Effects of Astragaloside IV on Uric Acid-Induced Pancreatic β-Cell Injury through PI3K/AKT Pathway Activation
Figure 5
Wortmannin intercepted the protective effect of AS-IV in UA-stimulated Min6 cells. (a) Min6 cells were stimulated with UA and AS-IV/wortmannin. Cells were divided into two groups: UA ± 25 μmol/l AS-IV and UA ± 25 μmol/l AS-IV ± 100 nM wortmannin. (b) Caspase-3 activity was determined in cells from different groups. (c) Flow cytometry analysis was employed to quantify cell apoptosis. (d) Quantification of apoptotic cells. Data are expressed as mean ± SD. Differences between groups were estimated using the unpaired Student's t-test. vs. UA + AS-IV-25.
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