LC-QTOF-MS Characterization, Antioxidant Activity, and In Vitro Toxicity of Medicinal Plants from the Tri-Than-Thip Remedy

Table 2

Metal chelating activity (MCA) and free radical scavenging capacities of different extracts of Tri-Than-Thip and three botanical extracts.

Extracts	MCA assay (IC50; mg/mL)	Radical scavenging properties (IC50; mg/mL)
		DPPH	ABTS	NBT
Tri-TT	0.02 ± 0.00	0.08 ± 0.00	0.02 ± 0.00	0.205 ± 0.057
P. dulce	0.01 ± 0.00^b	0.07 ± 0.00^a	0.01 ± 0.00^a	0.33 ± 0.23^b
C. fistula	0.02 ± 0.00^c	0.10 ± 0.00^a	0.01 ± 0.00^a	0.08 ± 0.02^a
F. benjamina	0.01 ± 0.00^a	1.69 ± 1.79^b	0.06 ± 0.01^b	1.06 ± 0.25^c

IC₅₀ of EDTA (a positive control) was 0.01 ± 0.00 mg/mL. The IC₅₀ values of Trolox obtained from the DPPH, ABTS, and NBT assays were 0.025, 0.020, and 0.025 mg/mL, respectively. ^a–cValues in the same column with different superscripts are significantly different . Extraction yield (g/100 g dried plant material).