Exploring the Effect and Mechanism of Si-Miao-Yong-An Decoction on Abdominal Aortic Aneurysm Based on Mice Experiment and Bioinformatics Analysis

Figure 1

Si-Miao-Yong-An decoction (SMYAD) inhibited 3, 4-benzopyrene (Bap)/angiotensin II (Ang II)-induced AAA in mice. (a) Timeline of experimental period. (b) Representative images of abdominal aorta from normal control group (control for short), Bap-/Ang II-treated + saline group (Bap + Ang II for short), Bap-/Ang II-treated + SMYAD-low-dose group (Bap + Ang II + SL for short), and Bap-/Ang II-treated + SMYAD-high-dose group (Bap + Ang II + SH for short). No obvious pathologic expansion or hematoma formation was observed in the control. The arrow indicated pathologic expansion and abdominal aortic aneurysm (AAA) formation in Bap + Ang II. And the pathologic expansion of abdominal aortas in Bap-/Ang II-treated mice was both alleviated by SMYAD-low dose and SMYAD-high dose. (c) The perimeter of selected abdominal aortas (μm) in control (n = 6), Bap + Ang II (n = 12), Bap + Ang II + SL (n = 12), and Bap + Ang II + SH (n = 12) was measured and recorded at Day 43. Data represented the ratio or mean ± SD. vs control, vs Bap + Ang II, vs Bap + Ang II + SH. (d) Incidences of AAA (%) in control (0/6), Bap + Ang II (8/12), Bap + Ang II + SL (3/12), Bap + Ang II + SH (2/12). vs control, vs Bap + Ang II, vs Bap + Ang II + SH. ((e) ×20) Representative photomicrographs of abdominal aortic tissues were evaluated by using haematoxylin-eosin (HE) staining. Elastin lamella was continuous and complete in control. The elastic lamella lost fine lines and instead degraded into loosely defined lines in Bap + Ang II, and the severity of elastin lamella destruction was partly attenuated by receiving both SMYAD-low dose and SMYAD-high dose. Scale bar, 20 μm.

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