Development of a Hepatoprotective Herbal Drug from Turnera diffusa
Table 3
Parameters of identity and activity for the different HM samples prepared.
Sample
Date of collection
Hepatodamianol content (mg/g sample)
Antioxidant activity (CE50μg/ml)
Flavonoid content (mg equivalent/g sample)
Hepatoprotection activity AST (IU/L)
Quercetin
Rutin
HM1
January 2015
16.95 ± 0.31
55.24 ± 7.68
80.11 ± 1.65
186.97 ± 4.01
37.33 ± 5.13
HM2
November 2015
18.42 ± 0.37
62.20 ± 7.97
68.62 ± 0.88
159.09 ± 2.15
38.66 ± 1.15
HM3
November 2014
25.73 ± 0.73
58.74 ± 7.86
52.54 ± 0.66
120.06 ± 1.61
38.66 ± 2.08
HM4
December 2015
15.98 ± 0.34
52.79 ± 6.42
68.18 ± 0.45
153.178 ± 1.10
38.00 ± 2.64
HM5
April 2016
19.33 ± 0.95
78.48 ± 9.09
57.81 ± 0.29
32.86 ± 0.72
33.00 ± 1.00
HM6
July 2015
11.66 ± 1.00
52.13 ± 4.03
88.42 ± 0.52
207.16 ± 1.26
38.33 ± 3.51
HM7
October 2015
13.40 ± 0.34
60.29 ± 8.00
64.68 ± 0.63
149.52 ± 1.53
36.33 ± 5.85
HM8
February 2016
8.76 ± 0.26
66.55 ± 8.69
47.76 ± 0.15
108.45 ± 0.36
39.00 ± 2.64
HM9
June 2015
20.99 ± 0.60
26.92 ± 0.48
162.58 ± 0.36
379.69 ± 0.89
41.33 ± 2.51
HM10
June 2015
7.91 ± 0.25
90.32 ± 1.89
49.36 ± 0.70
112.32 ± 1.69
45.66 ± 3.05
Quercetin
3.88 ± 0.16
Hepatodamianol
4.76 ± 0.2
20.23 ± 3.67
Silibinin
81.33 ± 6.02
Hepatoprotection model in vitro: HepG2 cells, CCl4 0.4% as damage agent, cells + CCl4: AST 111.33 ± 3.51, cells without CCl4: AST 0.66 ± 0.57. Cytotoxicity was assessed in Vero cells; n = 3. HM1 to HM10 are standardized extracts obtained from 10 different samples of Turnera diffusa with the procedure described.
