Root Extract of a Micropropagated Prunus africana Medicinal Plant Induced Apoptosis in Human Prostate Cancer Cells (PC-3) via Caspase-3 Activation

Figure 6

Apoptosis assay of PC-3 cells after 48 h treatment with PWS and PIR samples. (a) Apoptosis levels after annexin-V and PI staining of treated PC3-cells. (b) Apoptosis levels of treated PC-3 cells by flow cytometry (FACS) after annexin-V and PI staining (the upper left quadrant indicates necrotic cells (annexin V (−)/PI (+)), the upper right quadrant indicates late apoptotic cells (annexin V (+)/PI (+)), lower right quadrant indicates early apoptotic cells (annexin V (+)/PI (−)), and the lower left quadrant indicates healthy cells (annexin V (−)/PI (−)). (c) Dead cells of treated PC-3 cells when stained with trypan blue.

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