Mechanism of Long Noncoding RNA HOTAIR in Nucleus Pulposus Cell Autophagy and Apoptosis in Intervertebral Disc Degeneration

Figure 1

HOTAIR is highly expressed in NP cells of IDD patients. (a) The morphological changes of normal and degenerative NP cells were observed using an inverted phase-contrast microscope. (b) Normal and degenerative NP cells were observed by ICC and toluidine blue staining. (c) Collagen II and aggrecan mRNA expressions in normal and degenerative NP cells were detected using RT-qPCR. (d) MTT assay was used to detect the proliferation of normal and degenerative NP cells of P2 generation. (e) RT-qPCR was used to detect the expression of HOTAIR in normal and degenerative NP cells. The experiment was repeated three times, and the data were expressed as mean ± standard deviation. Data comparisons between two groups were analyzed using independent t-test. ; .

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