Research Article

Mechanism of Long Noncoding RNA HOTAIR in Nucleus Pulposus Cell Autophagy and Apoptosis in Intervertebral Disc Degeneration

Figure 4

miR-148a knockdown reverses the inhibition of si-HOTAIR on the autophagy and apoptosis of degenerative NP cells. miR-148a inhibitor/inhibitor NC was transfected into degenerative NP cells with si-HOTAIR treatment group (si-HOTAIR + in-NC group/si-HOTAIR + in-miR-148a group). (a) The expression of miR-148a in normal and degenerative NP cells was detected using RT-qPCR. (b) Levels of autophagy-related proteins (p62, LC3II/I, and Beclin-1) were detected using WB. (c) Apoptosis was detected using flow cytometry. The cell experiment was repeated three times, and the data were expressed as mean ± standard deviation. Data comparisons between two groups were analyzed using t-test. .
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