Mechanism of Long Noncoding RNA HOTAIR in Nucleus Pulposus Cell Autophagy and Apoptosis in Intervertebral Disc Degeneration

Figure 5

Silencing HOTAIR inhibits autophagy and apoptosis and improves pathological damage of NP cells in IDD rats. (a) RT-qPCR was used to detect the expression of HOTAIR in IVD tissues. (b) The pathological condition of NP tissues in each group was evaluated using HE staining and scored by histological evaluation. (c) WB was used to detect the levels of autophagy-related proteins (p62, LC3II/I, and Beclin-1) in IVD tissues. (d). RT-qPCR was used to detect the expression of miR-148a in IVD tissues. (e) TUNEL staining was used to detect apoptosis. (f) RT-qPCR was used to detect PTEN mRNA expression in IVD tissues. The experiment was repeated three times, and the data were expressed as mean ± standard deviation. Data comparisons among multigroups were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. .

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