Mechanism of Long Noncoding RNA HOTAIR in Nucleus Pulposus Cell Autophagy and Apoptosis in Intervertebral Disc Degeneration

Table 1

Primer sequences for RT-qPCR.

Gene	Primer
HOTAIR	F: 5′-CATTCTGCCCTGATTTCCG-3′
	R: 5′-ATCCGTTCCATTCCACTGCG-3′
miR-148a	F: 5′-ATGCTCAGTGCACTACAGAA-3′
	R: 5′-GTGCAGGGTCCGAGGT-3′
Aggrecan	F: 5′-TGAGCGGCAGCACTTTGAC-3′
	R: 5′-TGAGTACAGGAGGCTTGAGG-3′
Collagen II	F: 5′-TCCAGATGACCTTCCTACGC-3′
	R: 5′-TCCAGATGACCTTCCTACGC-3′
PTEN	F: 5′-CCATAACCCACCACAG-3′
	R: 5′-CAGTCCGTCCTTTC-3′
GAPDH	F: 5′-GGGAGCCAAAAGGGTCAT-3′
	R: 5′-GAGTCCTTCCACGATACCAA-3′
U6	F: 5′-CGCTTCGGCAGCACATATAC-3′
	R: 5′-AATATGGAACGCTTCACGA-3′