Mechanism of Huangqi Sanxian Decoction Inhibiting Osteoclast Differentiation Based on Network Pharmacology

Figure 13

HQSXD impairs RANKL-induced Akt phosphorylation. (a) BMMs were pretreated with HQSXD for 2 day and then stimulated with RANKL for the indicated times. Cell lysates were then subjected to western blot analysis for p-Akt, Akt and GAPDH. (b) Quantification of p-Akt protein expression levels was normalized to total Akt levels. ; n = 3.

(a)

(b)