The Reduction of Tau Hyperphosphorylation by Cornel Iridoid Glycosides Is Mediated by Their Influence on Calpain Activity

Figure 2

Effects of treatment with CIG on calpain activity. (a) CIG reduced calpain activity in P301S mice and MAPT cells. The expression of (b) calpain 1, (c) calpain 2, (d) spectrin, and GAPDH in P301S mice is shown. Mean ± SEM, n = 4; , compared with the nTg group; #, ##, compared with the P301S group. MAPT cells were treated with CIG for 24 hours. (e) The expression of (f) calpain 1, (g) calpain 2, (h) spectrin, and GAPDH in MAPT cells is shown. Mean ± SEM, n = 3; , compared with the N2a group; #, compared with the MAPT group. CIG (L): 50 μg/mL, CIG (M): 100 μg/mL, CIG (H): 200 μg/mL, CIG: cornel iridoid glycosides, MAPT: microtubule-associated protein tau, and GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

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