Antidiarrheal Effect of 80% Methanol Extract and Fractions of the Roasted Seed of Coffea arabica Linn (Rubiaceae) in Swiss Albino Mice

Table 3

Effects of 80% methanol crude extract and solvent fractions of seeds of Coffea arabica Linn on the gastrointestinal fluid accumulation in mice.

Group and dose	Volume of intestinal content (ml)	% inhibition	Weight of intestinal content (g)	% inhibition
Control	0.69 ± 0.11	—	0.73 ± 0.11	—
80ME 100	0.55 ± 0.05^b2	20.3	0.56 ± 0.05^b1	23.3
80ME 200	0.34 ± 0.08^a1	50.7	0.4 ± 0.08^a1	45.2
80ME 400	0.28 ± 0.03^a2	59.4	0.32 ± 0.03^a2	56.2
Lop 3	0.17 ± 0.02^a3	75.4	0.26 ± 0.04^a2	64.4
Control	0.62 ± 0.1	—	0.68 ± 0.1	—
HF 100	0.55 ± 0.03^b3	11.3	0.60 ± 0.03	11.8
HF 200	0.44 ± 0.04^b2	29	0.50 ± 0.04	26.5
HF 400	0.41 ± 0.03^b1	33.9	0.47 ± 0.03	30.9
Lop 3	0.16 ± 0.03^a3	74.2	0.21 ± 0.03^a3,c3,d2,e1	69.1
Control	0.64 ± 0.11	—	0.69 ± 0.11	—
EF 100	0.37 ± 0.03^a1	42.2	0.44 ± 0.04	36.2
EF 200	0.34 ± 0.02^a1	46.9	0.39 ± 0.02^a1	43.5
EF 400	0.33 ± 0.08^a1	48.4	0.36 ± 0.08^a1	47.8
Lop 3	0.17 ± 0.02^a3	73.4	0.21 ± 0.02^a3	69.6
Control	0.70 ± 0.08	—	0.74 ± 0.08	—
AF 100	0.54 ± 0.03	22.9	0.60 ± 0.04	18.9
AF 200	0.53 ± 0.02	24.3	0.57 ± 0.02	23
AF 400	0.52 ± 0.02^a1	25.7	0.56 ± 0.03^a1	24.3
Lop 3	0.17 ± 0.01^a3,c3,d3,e3	75.7	0.25 ± 0.01^a3,c3,d3,e3	66.2

All values are expressed as mean ± standard error of the mean (SEM) (n = 6); ^acompared to the control, ^bcompared to the standard, ^ccompared to 100 mg/kg, ^dcompared to 200 mg/kg, ^ecompared to 400 mg/kg, , , and ; 80ME = 80% hydromethanolic extract, HF = hexane fraction, EF = ethyl acetate fraction, AF = aqueous fraction, and Lop3 = loperamide 3 mg/kg. Controls received 10 ml/kg distilled water (for AF and 80ME) and 2% tween 80 (for EF and HF).