Antidiarrheal Effect of 80% Methanol Extract and Fractions of the Roasted Seed of Coffea arabica Linn (Rubiaceae) in Swiss Albino Mice
Table 3
Effects of 80% methanol crude extract and solvent fractions of seeds of Coffea arabica Linn on the gastrointestinal fluid accumulation in mice.
Group and dose
Volume of intestinal content (ml)
% inhibition
Weight of intestinal content (g)
% inhibition
Control
0.69 ± 0.11
—
0.73 ± 0.11
—
80ME 100
0.55 ± 0.05b2
20.3
0.56 ± 0.05b1
23.3
80ME 200
0.34 ± 0.08a1
50.7
0.4 ± 0.08a1
45.2
80ME 400
0.28 ± 0.03a2
59.4
0.32 ± 0.03a2
56.2
Lop 3
0.17 ± 0.02a3
75.4
0.26 ± 0.04a2
64.4
Control
0.62 ± 0.1
—
0.68 ± 0.1
—
HF 100
0.55 ± 0.03b3
11.3
0.60 ± 0.03
11.8
HF 200
0.44 ± 0.04b2
29
0.50 ± 0.04
26.5
HF 400
0.41 ± 0.03b1
33.9
0.47 ± 0.03
30.9
Lop 3
0.16 ± 0.03a3
74.2
0.21 ± 0.03a3,c3,d2,e1
69.1
Control
0.64 ± 0.11
—
0.69 ± 0.11
—
EF 100
0.37 ± 0.03a1
42.2
0.44 ± 0.04
36.2
EF 200
0.34 ± 0.02a1
46.9
0.39 ± 0.02a1
43.5
EF 400
0.33 ± 0.08a1
48.4
0.36 ± 0.08a1
47.8
Lop 3
0.17 ± 0.02a3
73.4
0.21 ± 0.02a3
69.6
Control
0.70 ± 0.08
—
0.74 ± 0.08
—
AF 100
0.54 ± 0.03
22.9
0.60 ± 0.04
18.9
AF 200
0.53 ± 0.02
24.3
0.57 ± 0.02
23
AF 400
0.52 ± 0.02a1
25.7
0.56 ± 0.03a1
24.3
Lop 3
0.17 ± 0.01a3,c3,d3,e3
75.7
0.25 ± 0.01a3,c3,d3,e3
66.2
All values are expressed as mean ± standard error of the mean (SEM) (n = 6); acompared to the control, bcompared to the standard, ccompared to 100 mg/kg, dcompared to 200 mg/kg, ecompared to 400 mg/kg, ,, and ; 80ME = 80% hydromethanolic extract, HF = hexane fraction, EF = ethyl acetate fraction, AF = aqueous fraction, and Lop3 = loperamide 3 mg/kg. Controls received 10 ml/kg distilled water (for AF and 80ME) and 2% tween 80 (for EF and HF).