Biochemical Characterization of a Thiol-Activated, Oxidation Stable Keratinase from Bacillus pumilus KS12

Figure 2

SDS-PAGE and zymogram analysis of purified keratinase (a) SDS PAGE, Lane 1: Protein molecular mass marker, Lane 2: Culture supernatant, Lane 3: Purified fraction eluted from DEAE Sepharose column (b) Zymogram.