Research Article
Biochemical Characterization of a Thiol-Activated, Oxidation Stable Keratinase from Bacillus pumilus KS12
Table 1
Purification scheme of keratinase.
| | Components | Total enzyme activity (U) | Total protein (mg) | Specific activity (U/mg) | Recovery (%) | Purification fold |
| | Lyophilized Culture broth | 2170.00 | 12.00 | 180.83 | 100.00 | 1.00 | | DEAE-Sepharose | 716.00 | 1.80 | 397.77 | 32.99 | 2.19 |
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