Biochemical Characterization of a Thiol-Activated, Oxidation Stable Keratinase from Bacillus pumilus KS12

Table 2

Effect of inhibitors on activity of keratinase.

S. no.	Concentration (mM)	Residual activity (%)
		PMSF	EDTA	DTT	𝛽-mercaptoethanol
(1)	1	5.00	74.00	120.00	240.00
(2)	5	0.00	70.00	158.00	609.00
(3)	10	0.00	70.00	203.00	876.00

Control without inhibitors was taken as 100% activity corresponded to 720 U/mL on feather keratin as substrate.