Biochemical Characterization of a Thiol-Activated, Oxidation Stable Keratinase from <i>Bacillus pumilus</i> KS12

<table class="table-group" id="tab5"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td align="left">Substrates</td><td align="center"><span style="width: 21.9375px;"><svg height="15.25" id="M57" style="vertical-align:-3.27605pt" version="1.1" viewbox="0 0 21.9375 15.25" width="21.9375" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg></span></td><td align="center"><span style="width: 37.912498px;"><svg height="15.4" id="M58" style="vertical-align:-3.3907pt" version="1.1" viewbox="0 0 37.912498 15.4" width="37.912498" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><path d="M667 0h-153l-248 291q-28 32 -54 27v-190q0 -65 14.5 -80t77.5 -20v-28h-260v28q58 5 71 20t13 80v392q0 48 -6 66.5t-22 25.5t-59 10v28h258v-28q-59 -5 -73 -20.5t-14 -81.5v-175q29 6 70 45q101 97 167 178q23 27 20 37t-36 14l-23 3v28h240v-28q-57 -5 -86 -18.5
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<g transform="matrix(.012,-0,0,-.012,11.5,15.187)"><path d="M390 111l17 -21q-34 -45 -80 -73.5t-89 -28.5q-91 0 -146 62t-55 147q0 118 101 195q74 57 149 57h1q59 0 90 -27q16 -14 16 -30q0 -15 -12 -29t-21 -14q-8 0 -19 11q-44 41 -101 41q-52 0 -87.5 -42.5t-35.5 -117.5q0 -49 15 -87t39 -58t49 -30t48 -10q33 0 60.5 12
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</svg>  (min<sup>-1</sup>)</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td align="left">Casein</td><td align="center">0.25 mg/mL</td><td align="center">0.75 <svg height="12.175" id="M60" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 9.5625 12.175" width="9.5625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><path d="M538 96q-38 -45 -84 -76.5t-71 -31.5q-44 0 -15 117q12 49 18 89h-2q-99 -128 -163 -178q-36 -28 -70 -28q-26 0 -44 38h-2q-16 -69 -16 -129q0 -43 11 -75.5t24 -42.5l1 -6q-7 -12 -24.5 -23t-37.5 -11q-40 0 -40 76q0 52 35 202l94 407l78 24l5 -5q-32 -122 -72 -310
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</svg>g/mL/min</td><td align="center">3.0</td></tr><tr><td align="left">N-Suc-Ala-Ala-Pro-Phe-pNA</td><td align="center">0.1 mM</td><td align="center">2.0 <svg height="12.175" id="M61" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 9.5625 12.175" width="9.5625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>moles/mL/min</td><td align="center">20.0</td></tr><tr><td align="left" rowspan="3">N-Ala-Ala-Pro-Leu-pNA</td><td align="center"></td><td align="center">3.0 <svg height="12.175" id="M62" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 9.5625 12.175" width="9.5625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><path d="M538 96q-38 -45 -84 -76.5t-71 -31.5q-44 0 -15 117q12 49 18 89h-2q-99 -128 -163 -178q-36 -28 -70 -28q-26 0 -44 38h-2q-16 -69 -16 -129q0 -43 11 -75.5t24 -42.5l1 -6q-7 -12 -24.5 -23t-37.5 -11q-40 0 -40 76q0 52 35 202l94 407l78 24l5 -5q-32 -122 -72 -310
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</svg>moles/mL/min</td><td align="center">12.0</td></tr><tr><td align="center">0.25  mM</td><td align="left"></td><td align="left"></td></tr><tr><td align="left"></td><td align="left"></td><td align="left"></td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Kinetic parameters of purified keratinase on casein and <i>p</i>NA.

Enzyme Research

tab5

Table 5

Table 5: Biochemical Characterization of a Thiol-Activated, Oxidation Stable Keratinase from <i>Bacillus pumilus</i> KS12 

Enzyme Research

Biochemical Characterization of a Thiol-Activated, Oxidation Stable Keratinase from Bacillus pumilus KS12

Table 5