Purification, Characterization, and Effect of Thiol Compounds on Activity of the Erwinia carotovora L-Asparaginase

Table 1

L-asparaginase purification summary.

Purification steps	Total activity (IU)	Total Protein (mg)	Specific activity (IU/mg)	Purification fold	Recovery (%)
Spheroplast suspension	6800	5000	1.36	1.0	100
Ammonium sulfate precipitation	5000	1400	3.57	2.62	73
Sephadex G-100 gel filtration	4100	248.9	16.4	12.11	60
CM Cellulose	3172	10.2	310.9	228.6	46
DEAE sephadex	2482	2.4	1034	752.9	36.5