Purification, Characterization, and Effect of Thiol Compounds on Activity of the Erwinia carotovora L-Asparaginase

Table 3

Kinetic constants of partially purified asparaginase.

Substrate	Linear regression (Lineweaver-Burkplot)			Nonlinear regression (Michaelis-Menten equation)
	K𝑚	Vmax	Vmax/K𝑚	K𝑚	Vmax	Vmax/K𝑚
	(mM)	(𝜇moles mg^-1min^-1)		(mM)	(𝜇moles mg^-1min^-1)
L-asparagine	0.096	1632.6	17,006	0.098	1666.7	17,007
L-glutamine	2.86	4.08	1.426	3.04	4.74	1.559