Research Article

Enzyme Inhibition by Molluscicidal Components of Myristica fragrans Houtt. in the Nervous Tissue of Snail Lymnaea acuminata

Table 1

In vivo effect of 24 and 96 h exposure to sublethal concentration of trimyristin and myristicin on acetylcholinesterase (AChE), acid phosphatase (ACP), and alkaline phosphatase (ALP) activities in the nervous tissue of Lymnaea acuminata.

EnzymeTreatmentEnzyme activity
Control40% of 24 h LC5080% of 24 h LC5040% of 96 h LC5080% of 96 h LC50

AChETrimyristin0.73 ± 0.0 (100)0.66 ± 0.0* (90)0.60 ± 0.0* (82)0.53 ± 0.0*(73)0.46 ± 0.0* (63)
Myristicin0.73 ± 0.0 (100)0.57 ± 0.0* (78)0.52 ± 0.0* (71)0.45± 0.0* (62)0.36 ± 0.0* (49)
ACPTrimyristin32.3 ± 0.1 (100)28.0 ± 0.1*(87)25.7 ± 0.3* (80)23.7 ± 0.0* (73)20.7 ± 0.2* (64)
Myristicin32.3 ± 0.1 (100)26.0 ± 0.3* (80)21.5 ± 0.0* (67)17.9 ± 0.1* (55)14.2 ± 0.1* (44)
ALPTrimyristin30.0 ± 0.1 (100)27.5 ± 0.1* (92)25.0 ± 0.1* (84)21.4 ± 0.1* (71)18.2 ± 0.2* (61)
Myristicin30.0 ± 0.1 (100)22.8 ± 0.1* (76)19.8 ± 0.1* (66)17.7 ± 0.1* (59)15.7 ± 0.2* (52)

Values are mean ±SE of six replicates. Values in parentheses indicate per cent of enzyme activity with control taken as 100%.
Concentrations (w/v) have been expressed as final concentration in aquarium water. Acetylcholinesterase activity, mole “SH” hydrolyzed min-1 mg-1 protein.
Acid phosphatase activity, mole substrate hydrolyzed 30 min-1 mg-1 protein.
Alkaline phosphatase activity, mole substrate hydrolyzed 30 min-1 mg-1 protein.
*Significant (P < .05) when t-test was used for locating difference between treated and control group of animals.