Characterization of Activity of a Potential Food-Grade Leucine Aminopeptidase from Kiwifruit
Figure 6
Elution profile from a DEAE cellulose column of aminopeptidase (AP) activity and protein content in a concentrated fraction of ammonium sulfate precipitation of crude extracts of A. deliciosa fruit. The first 30 fractions were eluted with 10 mM potassium phosphate buffer (pH 8) supplemented with 10% glycerol. The next fractions were eluted with a linear gradient of 0 to 1.0 M KCl in the same buffer. One unit of enzyme activity was defined as a change in one unit of absorbance at 410 nm per h at . Protein content was measured at 280 nm.
