Enzyme Research

Enzyme Research / 2010 / Article / Tab 1

Research Article

A Simple Enzymatic Method for Production of a Wide Variety of D-Amino Acids Using L-Amino Acid Oxidase from Rhodococcus sp. AIU Z-35-1

Table 1

Analysis of oxidation products from L-amino acids. Twenty micromoles of each L-amino acid was incubated with 15.5 10-3 U of L-AAO under standard conditions of L-amino acid oxidation, and the reaction mixture was applied to HPLC with a TSKgel DEAE-5PW column. Molecular mass of reaction products was analyzed by an HCT Ultra mass spectrometric instrument.

L-Amino acidElution time (min)Reaction product
L-Amino acidProductsMolecular mass (product name)

L-Homoserine3.315.8118.1 (2-oxo-4-hydroxybutyric acid)
L-Leucine3.218.3130.0 (2-oxo-4-methylvaleric acid)
L-Lysine2.74.8145.1 (2-oxo-6-aminohexanoic acid)
L-Glutamine3.315.1145.1 (2-oxo-4-carbamoylbutanoic acid)
L-Arginine2.33.3173.1 (2-oxo-5-guanidinopentanoic acid)
L-Glutamic acid-Na14.618.5190.0 (2-oxoglutaric acid)
L-Citrulline3.214.9174.0 (2-oxo-5-ureidovaleric acid)
15.8174.0 (pyrrolidine-1-carbamyl-2-hydroxy-2-carboxylic acid)
L-Phenylaranine3.524.9164.1 (2-oxo-3-phenylpropionic acid)
L-Histidine3.017.9154.3 (2-oxo-4-imidazolepropionic acid)
Nε-Acetyl-L-lysine3.215.5187.1 (2-oxo-6-acetylaminohexanoic acid)