A Simple Enzymatic Method for Production of a Wide Variety of D-Amino Acids Using L-Amino Acid Oxidase from Rhodococcus sp. AIU Z-35-1
Analysis of oxidation products from L-amino acids. Twenty micromoles of each L-amino acid was incubated with 15.5 10-3 U of L-AAO under standard conditions of L-amino acid oxidation, and the reaction mixture was applied to HPLC with a TSKgel DEAE-5PW column. Molecular mass of reaction products was analyzed by an HCT Ultra mass spectrometric instrument.