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Enzyme Research
Volume 2010, Article ID 703824, 7 pages
Research Article

The Use of HRP in Decolorization of Reactive Dyes and Toxicological Evaluation of Their Products

1Biocatalysis Laboratory, Catalysis Division, National Institute of Technology, Ministry of Science and Technology, Avenue Venezuela 82, Sala 302, 20081-312 Rio de Janeiro, RJ, Brazil
2Department of Technology and Biochemical Processes, Chemistry Institute, State University of Rio de Janeiro, Rua São Francisco Xavier, 524, Lab 310, 20550-900 Rio de Janeiro, RJ, Brazil
3Department of Biochemistry, Federal University of Juiz de Fora, 36036-330 Juiz de Fora, MG, Brazil

Received 9 August 2010; Revised 9 December 2010; Accepted 23 December 2010

Academic Editor: Denise M. G. Freire

Copyright © 2010 Michelle Reis da Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This work studied the potential use of horseradish peroxidase (HRP) in the decolorization of the following textile dyes: Drimarene Blue X-3LR (DMBLR), Drimarene Blue X-BLN (DMBBLN), Drimarene Rubinol X-3LR (DMR), and Drimarene Blue CL-R (RBBR). Dyes were individually tested in the reaction media containing 120 mg⋅L-1, considering the following parameters: temperature (20–45°C), H2O2 concentration (0–4.44 mmol⋅L-1), and reaction time (5 minutes, 1 and 24 h). The following conditions: 35°C, 0.55 mmol⋅L-1, and 1h, provided the best set of results of color removal for DMBLR (99%), DMBBLN (77%), DMR (94%), and RBBR (97%). It should be mentioned that only 5 minutes of reaction was enough to obtain 96% of decolorization for DMBLR and RBBR. After the decolorization reactions of DMBLR, DMR, and RBBR, it was possible to observe the reduction of Artemia salina mortality and the no significant increase in toxicity for the products generated from DMBBLN.