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Enzyme Research
Volume 2010, Article ID 824763, 7 pages
Research Article

Hyperbaric Oxygen Exposure Reduces Age-Related Decrease in Oxidative Capacity of the Tibialis Anterior Muscle in Mice

1Beauty Care Research Laboratories, Kao Corporation, Tokyo 131-8501, Japan
2Laboratory of Neurochemistry, Graduate School of Human and Environmental Studies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
3Division of Rehabilitation Sciences, Kobe University Graduate School of Health Sciences, Kobe 654-0142, Japan
4Biological Science Laboratories, Kao Corporation, Tochigi 321-3497, Japan
5Department of Physical Therapy, Faculty of Health Care Science, Himeji Dokkyo University, Himeji 670-8524, Japan

Received 13 June 2009; Revised 10 October 2009; Accepted 29 October 2009

Academic Editor: Vasu Appanna

Copyright © 2010 Takahiro Nishizaka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effects of exposure to hyperbaric oxygen on the oxidative capacity of the skeletal muscles in mice at different ages were investigated. We exposed 5-, 34-, 55-, and 88-week-old mice to 36% oxygen at 950 mmHg for 6 hours per day for 2 weeks. The activities of succinate dehydrogenase (SDH), which is a mitochondrial marker enzyme, of the tibialis anterior muscle in hyperbaric mice were compared with those in age-matched mice under normobaric conditions (21% oxygen at 760 mmHg). Furthermore, the SDH activities of type IIA and type IIB fibers in the muscle were determined using quantitative histochemical analysis. The SDH activity of the muscle in normobaric mice decreased with age. Similar results were observed in both type IIA and type IIB fibers in the muscle. The decrease in the SDH activity of the muscle was reduced in hyperbaric mice at 57 and 90 weeks. The decreased SDH activities of type IIA and type IIB fibers were reduced in hyperbaric mice at 90 weeks and at 57 and 90 weeks, respectively. We conclude that exposure to hyperbaric oxygen used in this study reduces the age-related decrease in the oxidative capacity of skeletal muscles.