Optimization of a Cytochrome-P450-Monooxygenase-1A-Mediated EROD Assay in the Cape Hake Species Merluccius capensis and Merluccius paradoxus (Pisces)

<table>Dot blot analysis of CYP1A in different microsomal fractions in Cape hake. (a): Crude (9.600 μg/μL), (b): Supernatant 1 (0.058 μg/2 μL); (c): Pellet 1 (3.128 μg/2 μL); (d): Supernatant 2 (5.442 μg/2 μL); (e): Pellet 2 (0.028 μg/2 μL); (f) Negative control: Homogenization buffer. Two μL of protein sample from each fraction were spotted onto the nitrocellulose membrane.</table>

Enzyme Research

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Figure 2

Figure 2: Optimization of a Cytochrome-P450-Monooxygenase-1A-Mediated EROD Assay in the Cape Hake Species Merluccius capensis and Merluccius paradoxus (Pisces)

Figure 2 | Optimization of a Cytochrome-P450-Monooxygenase-1A-Mediated EROD Assay in the Cape Hake Species  Merluccius capensis and Merluccius paradoxus (Pisces) 