Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus

Table 1

Purification of recombinant PfuDHQS from E. coli.

Step	Total protein (mg)	Total enzyme activity (U)	Calculated specific activity (U mg^-1)	Yield (%)	Approximate purity
Total crude	46	18	0.17	100	1
Heat treated	6.7	13	1.9	72	11
Anion exchange	3.2	11	3.2	59	19