Production and Partial Characterization of Cellulases from Trichoderma sp. IS-05 Isolated from Sandy Coastal Plains of Northeast Brazil

Table 1

Values of independent variables (WB concentration X₁ and peptone concentration X₂, resp.) used in CCRD, showing the values observed and predicted by the mathematical model for cellulase production by Trichoderma sp. IS-05.

Run	Coded setting levels		Actual levels (% w/v)		Cellulase activity (U L^−1)
	𝑋1	𝑋2	𝑋1	𝑋2	O	P
1	−1	−1	2.0	0.05	8.2	102.2
2	+1	−1	4.0	0.05	447.3	463.0
3	−1	+1	2.0	0.25	83.6	102.2
4	+1	+1	4.0	0.25	563.6	463.1
5	−1.41	0	1.59	0.15	21.3	−55.7
6	+1.41	0	4.41	0.15	389.9	453.2
7	0	−1.41	3.0	0.009	465.3	366.6
8	0	+1.41	3.0	0.291	281.8	366.7
9	0	0	3.0	0.15	275.3	285.1
10	0	0	3.0	0.15	288.4	285.1
11	0	0	3.0	0.15	291.6	285.1

Results are the mean of two experiments, O = observed; P = predict.