Research Article
Decolorization of Solophenyl Red 3BL Polyazo Dye by Laccase-Mediator System: Optimization through Response Surface Methodology
Table 2
Experimental conditions of the Box-Behnken design in coded and natural variables and the corresponding experimental and theoretical responses.
| | Run N° | | | | Enzyme | Mediator | Time | Measured decolorization | Estimated decolorization | | | | | | (U/mL) | (μM) | (h) | (%) | (%) |
| | 1 | −1.00 | −1.00 | 0.00 | 0.5 | 3.0 | 12.5 | 18.36 | 21.79 | | 2 | 1.00 | −1.00 | 0.00 | 2.5 | 3.0 | 12.5 | 26.42 | 25.16 | | 3 | −1.00 | 1.00 | 0.00 | 0.5 | 30.0 | 12.5 | 76.17 | 77.43 | | 4 | 1.00 | 1.00 | 0.00 | 2.5 | 30.0 | 12.5 | 66.15 | 62.72 | | 5 | −1.00 | 0.00 | −1.00 | 0.5 | 16.5 | 1.0 | 36.72 | 36.28 | | 6 | 1.00 | 0.00 | −1.00 | 2.5 | 16.5 | 1.0 | 31.52 | 35.78 | | 7 | −1.00 | 0.00 | 1.00 | 0.5 | 16.5 | 24.0 | 60.87 | 56.62 | | 8 | 1.00 | 0.00 | 1.00 | 2.5 | 16.5 | 24.0 | 45.34 | 45.78 | | 9 | 0.00 | −1.00 | −1.00 | 1.5 | 3.0 | 1.0 | 12.47 | 9.48 | | 10 | 0.00 | 1.00 | −1.00 | 1.5 | 30.0 | 1.0 | 54.59 | 53.77 | | 11 | 0.00 | −1.00 | 1.00 | 1.5 | 3.0 | 24.0 | 21.51 | 22.33 | | 12 | 0.00 | 1.00 | 1.00 | 1.5 | 30.0 | 24.0 | 68.25 | 71.24 |
| | Center points | 13 | 0.00 | 0.00 | 0.00 | 1.5 | 16.5 | 12.5 | 60.44 | 55.75 | | 14 | 0.00 | 0.00 | 0.00 | 1.5 | 16.5 | 12.5 | 55.71 | 55.75 | | 15 | 0.00 | 0.00 | 0.00 | 1.5 | 16.5 | 12.5 | 51.59 | 55.75 | | 16 | 0.00 | 0.00 | 0.00 | 1.5 | 16.5 | 12.5 | 53.68 | 55.75 | | 17 | 0.00 | 0.00 | 0.00 | 1.5 | 16.5 | 12.5 | 57.32 | 55.75 |
| | Check points | 18 | −0.40 | −0.25 | −0.17 | 1.1 | 13.1 | 10.5 | 51.59 | 48.12 | | 19 | 0.40 | −0.25 | −0.17 | 1.9 | 13.1 | 10.5 | 49.82 | 47.12 | | 20 | 0.00 | 0.45 | −0.17 | 1.5 | 22.6 | 10.5 | 65.24 | 63.22 | | 21 | 0.00 | 0.00 | −0.17 | 1.5 | 16.5 | 10.5 | 51.65 | 54.17 |
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