Enzyme Research

Enzyme Research / 2011 / Article / Tab 2

Research Article

Decolorization of Solophenyl Red 3BL Polyazo Dye by Laccase-Mediator System: Optimization through Response Surface Methodology

Table 2

Experimental conditions of the Box-Behnken design in coded and natural variables and the corresponding experimental and theoretical responses.

Run N° EnzymeMediatorTimeMeasured decolorizationEstimated decolorization
(U/mL)(μM)(h)(%)(%)

1−1.00−1.000.000.53.012.518.3621.79
21.00−1.000.002.53.012.526.4225.16
3−1.001.000.000.530.012.576.1777.43
41.001.000.002.530.012.566.1562.72
5−1.000.00−1.000.516.51.036.7236.28
61.000.00−1.002.516.51.031.5235.78
7−1.000.001.000.516.524.060.8756.62
81.000.001.002.516.524.045.3445.78
90.00−1.00−1.001.53.01.012.479.48
100.001.00−1.001.530.01.054.5953.77
110.00−1.001.001.53.024.021.5122.33
120.001.001.001.530.024.068.2571.24

Center points130.000.000.001.516.512.560.4455.75
140.000.000.001.516.512.555.7155.75
150.000.000.001.516.512.551.5955.75
160.000.000.001.516.512.553.6855.75
170.000.000.001.516.512.557.3255.75

Check points18−0.40−0.25−0.171.113.110.551.5948.12
190.40−0.25−0.171.913.110.549.8247.12
200.000.45−0.171.522.610.565.2463.22
210.000.00−0.171.516.510.551.6554.17