Research Article

Effect of Culture Conditions on the Production of an Extracellular Protease by Bacillus sp. Isolated from Soil Sample of Lavizan Jungle Park

Table 2

Effect of nitrogen source on Bacillus sp. strain CR-179 growth and protease activity. The culture density and extracellular protease activity were determined during 24 h incubation at 45°C and at initial pH 8.

Nitrogen sourceCulture density (OD 600 nm)Maximum enzyme activity (U/mL)

Corn steep liquor1.410336.233
Whey protein1.114239.870
Beef extract1.383203.246
Yeast extract1.391308.961
Tryptone0.954190
Peptone1.030119.350
Urea0.66646.883