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Enzyme Research
Volume 2011 (2011), Article ID 289206, 6 pages
http://dx.doi.org/10.4061/2011/289206
Research Article

Biotechnological Potential of Agro-Industrial Wastes as a Carbon Source to Thermostable Polygalacturonase Production in Aspergillus niveus

1Biochemistry and Immunology Department, Ribeirão Preto School of Medicine, São Paulo University, Avenue Bandeirantes, 3900, 14049-900 Ribeirão Preto, SP, Brazil
2Biology Department, Philosophy, Ribeirão Preto School of Philosophy, Sciences and Literature, São Paulo University, Avenue Bandeirantes, 3900, 14040-901 Ribeirão Preto, SP, Brazil

Received 27 October 2010; Revised 15 March 2011; Accepted 23 April 2011

Academic Editor: Richard John Ward

Copyright © 2011 Alexandre Maller et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Agro-industrial wastes are mainly composed of complex polysaccharides that might serve as nutrients for microbial growth and production of enzymes. The aim of this work was to study polygalacturonase (PG) production by Aspergillus niveus cultured on liquid or solid media supplemented with agro-industrial wastes. Submerged fermentation (SbmF) was tested using Czapeck media supplemented with 28 different carbon sources. Among these, orange peel was the best PG inducer. On the other hand, for solid state fermentation (SSF), lemon peel was the best inducer. By comparing SbmF with SSF, both supplemented with lemon peel, it was observed that PG levels were 4.4-fold higher under SSF. Maximum PG activity was observed at 55C and pH 4.0. The enzyme was stable at 60C for 90 min and at pH 3.0–5.0. The properties of this enzyme, produced on inexpensive fermentation substrates, were interesting and suggested several biotechnological applications.