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Enzyme Research
Volume 2011, Article ID 316939, 7 pages
http://dx.doi.org/10.4061/2011/316939
Research Article

Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100

1Laboratório de Biologia Molecular, Programa de Biotecnologia e Biologia Molecular, IBqM, UFRJ, 21941-902 Rio de Janeiro, RJ, Brazil
2Laboratório de Biotecnologia Microbiana, Departamento de Bioquímica, IQ, UFRJ, 21941-909 Rio de Janeiro, RJ, Brazil
3Laboratório de Microbiologia Molecular e Proteínas, Departamento de Bioquímica, IQ, UFRJ, 21941-909 Rio de Janeiro, RJ, Brazil
4Laboratório de Bioprocessos, Programa de Engenharia Química, COPPE, UFRJ, 21945-970 Rio de Janeiro, RJ, Brazil

Received 14 January 2011; Revised 5 April 2011; Accepted 3 May 2011

Academic Editor: G. Viniegra-Gonzalez

Copyright © 2011 Sylvia Maria Campbell Alquéres et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to Cite this Article

Sylvia Maria Campbell Alquéres, Roberta Vieira Branco, Denise Maria Guimarães Freire, Tito Lívio Moitinho Alves, Orlando Bonifácio Martins, and Rodrigo Volcan Almeida, “Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100,” Enzyme Research, vol. 2011, Article ID 316939, 7 pages, 2011. https://doi.org/10.4061/2011/316939.