Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100
Figure 2
(a) Effects of temperature on lipase activity. The purified lipases, TRX-PF2001Δ60 and PF2001Δ60, were assayed at temperatures ranging from 50 to 80°C, in 50 mM phosphate buffer, pH 7.0. (b) Effects of pH on lipase activity. The purified lipases, TRX-PF2001Δ60 and PF2001Δ60, were assayed at pH ranging from 5.0 to 9.0, in 100 mM BIS-TRIS-propane buffer at 70°C. All assays were carried out without Triton X-100.
