Research Article

Application of Asymetrical and Hoke Designs for Optimization of Laccase Production by the White-Rot Fungus Fomes fomentarius in Solid-State Fermentation

Table 2

Experimental conditions of the screening design and the corresponding responses.

Run
no.
M/S
ratio
(ml/gs)
MgSO4
(mg/gs)
CaCl2
(mg/gs)
Moisturizing
—agent
Inoculum
size
(discs)
Glucose
(mg/gs)
Ammonium
tartrate
(mg/gs)
CuSO4
(mg/gs)
Measured and estimated laccase activities (U/gds)
4
days
7
days
10
days
13
days
16
days

130.30.3CPB40002.16
1.88
37.00
38.68
2.88
9.93
10.00
9.54
6.60
10.26
230.30.3SAB4305.50.752.74
2.65
19.62
20.09
1.56
0.04
1.56
1.37
0.52
0.41
330.30.3CPB860111.52.74
2.65
39.92
40.39
13.60
11.99
17.04
16.85
5.30
4.37
430.30.3SAB80003.66
4.12
45.16
42.52
9.16
5.31
3.78
4.62
3.46
1.66
560.33CPB4301103.60
3.92
61.52
58.14
63.48
63.38
74.60
73.93
85.08
81.99
660.33SAB4001.54.70
5.04
63.48
61.83
69.38
78.32
96.86
93.51
108.64
107.09
760.33CPB8000.756.60
6.08
64.14
66.74
103.40
91.25
81.16
84.12
101.44
101.13
860.33SAB8605.505.16
5.02
57.60
60.02
61.52
64.82
67.40
68.45
65.44
70.38
9333CPB46000.751.50
1.88
36.00
34.83
7.98
12.98
2.34
1.70
2.02
3.28
10333SAB401103.26
2.80
37.96
42.77
15.96
8.91
7.46
10.46
0.26
4.29
11333CPB805.504.50
4.78
51.70
47.84
15.44
19.28
9.42
6.04
9.28
3.39
12333SAB83001.56.40
6.20
40.58
40.80
1.56
0.24
1.30
2.31
2.16
2.76
13630.3CPB405.51.54.90
4.85
56.94
57.90
99.48
93.93
87.70
90.22
92.28
94.16
14630.3SAB460003.60
3.45
55.62
53.89
70.68
63.98
61.52
61.30
66.76
61.49
15630.3CPB830006.60
6.57
58.24
60.92
64.80
68.30
57.60
57.44
60.20
63.61
16630.3SAB80110.755.94
6.17
63.48
61.57
83.12
91.87
82.46
80.32
80.50
80.48