Enzyme Research / 2011 / Article / Tab 2 / Research Article
Application of Asymetrical and Hoke Designs for Optimization of Laccase Production by the White-Rot Fungus Fomes fomentarius in Solid-State Fermentation Table 2 Experimental conditions of the screening design and the corresponding responses.
Run no. M/S ratio (ml/gs) MgSO4 (mg/gs) CaCl2 (mg/gs) Moisturizing —agent Inoculum size (discs) Glucose (mg/gs) Ammonium tartrate (mg/gs) CuSO4 (mg/gs)
Measured and estimated laccase activities (U/gds) 4 days 7 days 10 days 13 days 16 days 1 3 0.3 0.3 CPB 4 0 0 0 2.161.88 37.0038.68 2.889.93 10.009.54 6.6010.26 2 3 0.3 0.3 SAB 4 30 5.5 0.75 2.742.65 19.6220.09 1.56 −0.04 1.561.37 0.52 −0.41 3 3 0.3 0.3 CPB 8 60 11 1.5 2.742.65 39.9240.39 13.6011.99 17.0416.85 5.304.37 4 3 0.3 0.3 SAB 8 0 0 0 3.664.12 45.1642.52 9.165.31 3.784.62 3.461.66 5 6 0.3 3 CPB 4 30 11 0 3.603.92 61.5258.14 63.4863.38 74.6073.93 85.0881.99 6 6 0.3 3 SAB 4 0 0 1.5 4.705.04 63.4861.83 69.3878.32 96.8693.51 108.64107.09 7 6 0.3 3 CPB 8 0 0 0.75 6.606.08 64.1466.74 103.4091.25 81.1684.12 101.44101.13 8 6 0.3 3 SAB 8 60 5.5 0 5.165.02 57.6060.02 61.5264.82 67.4068.45 65.4470.38 9 3 3 3 CPB 4 60 0 0.75 1.501.88 36.0034.83 7.9812.98 2.341.70 2.023.28 10 3 3 3 SAB 4 0 11 0 3.262.80 37.9642.77 15.968.91 7.4610.46 0.264.29 11 3 3 3 CPB 8 0 5.5 0 4.504.78 51.7047.84 15.4419.28 9.426.04 9.283.39 12 3 3 3 SAB 8 30 0 1.5 6.406.20 40.5840.80 1.56 −0.24 1.302.31 2.162.76 13 6 3 0.3 CPB 4 0 5.5 1.5 4.904.85 56.9457.90 99.4893.93 87.7090.22 92.2894.16 14 6 3 0.3 SAB 4 60 0 0 3.603.45 55.6253.89 70.6863.98 61.5261.30 66.7661.49 15 6 3 0.3 CPB 8 30 0 0 6.606.57 58.2460.92 64.8068.30 57.6057.44 60.2063.61 16 6 3 0.3 SAB 8 0 11 0.75 5.946.17 63.4861.57 83.1291.87 82.4680.32 80.5080.48