Enzyme Research

Research Article

Application of Asymetrical and Hoke Designs for Optimization of Laccase Production by the White-Rot Fungus Fomes fomentarius in Solid-State Fermentation

Table 4

Experimental conditions of the Hoke design and the corresponding responses.


Run no.					CaCl₂ (mg/gds)	Inoculum size (Discs*)	CuSO₄ (mg/gds)	Incubation time (days)	Measured and estimated laccase activities (U/gds)

1	−1.00000	−1.00000	−1.00000	−1.00000	0.5	3	0.5	4	6.72	5.02
2	−1.00000	0.00000	0.00000	0.00000	0.5	6	1.5	10	121.00	119.93
3	0.00000	−1.00000	0.00000	0.00000	3.0	3	1.5	10	115.90	122.34
4	0.00000	0.00000	−1.00000	0.00000	3.0	6	0.5	10	101.12	110.96
5	0.00000	0.00000	0.00000	−1.00000	3.0	6	1.5	4	8.56	5.49
6	1.00000	1.00000	1.00000	−1.00000	5.5	9	2.5	4	11.60	14.77
7	1.00000	1.00000	−1.00000	1.00000	5.5	9	0.5	16	89.32	86.04
8	1.00000	−1.00000	1.00000	1.00000	5.5	3	2.5	16	138.24	136.66
9	−1.00000	1.00000	1.00000	1.00000	0.5	9	2.5	16	103.90	106.07
10	1.00000	1.00000	−1.00000	−1.00000	5.5	9	0.5	4	12.50	11.84
11	1.00000	−1.00000	1.00000	−1.00000	5.5	3	2.5	4	6.28	6.47
12	1.00000	−1.00000	−1.00000	1.00000	5.5	3	0.5	16	104.16	101.12
13	−1.00000	1.00000	1.00000	−1.00000	0.5	9	2.5	4	8.42	10.48
14	−1.00000	1.00000	−1.00000	1.00000	0.5	9	0.5	16	80.12	78.96
15	−1.00000	−1.00000	1.00000	1.00000	0.5	3	2.5	16	121.84	121.53
16	1.00000	1.00000	1.00000	0.00000	5.5	9	2.5	10	139.20	133.46
17	1.00000	1.00000	0.00000	1.00000	5.5	9	1.5	16	97.26	104.42
18	1.00000	0.00000	1.00000	1.00000	5.5	6	2.5	16	123.88	127.65
19	0.00000	1.00000	1.00000	1.00000	3.0	9	2.5	16	113.96	110.22

20	0.00000	0.00000	0.00000	0.00000	3.0	6	1.5	10	128.18	119.84
21	0.00000	0.00000	0.00000	0.00000	3.0	6	1.5	10	120.36	119.84
22	0.00000	0.00000	0.00000	0.00000	3.0	6	1.5	10	117.46	119.84
23	0.00000	0.00000	0.00000	0.00000	3.0	6	1.5	10	122.82	119.84

24	−0.39528	−0.22822	−0.16137	0.00000	2.0	5	1.3	10	122.28	118.35
25	0.39528	−0.22822	−0.16137	0.00000	4.0	5	1.3	10	120.42	120.51
26	0.00000	0.45644	−0.16137	0.00000	3.0	7	1.3	10	113.70	118.07
27	0.00000	0.00000	0.48412	0.00000	3.0	6	2.0	10	129.30	123.33
28	0.00000	0.00000	0.00000	0.50000	3.0	6	1.5	13	125.42	127.66

* diameter, 6 mm.