Laccase-Based CLEAs: Chitosan as a Novel Cross-Linking Agent

Table 3

Specific activity, thermal stability, and total amount of ABTS oxidized by all the prepared samples.

Sample	Specific activity* (U/g)	Thermal stability^*, a (%)	Aymard-Belarbi parameters			Half-life (h)	Amount of ABTS oxidized in 24 h under denaturing conditions (mmol/U)
			C	α	β
CLEA-0.2-200	298 ± 44	7.9 ± 1.2	0,71	0,15	1,78	2.36	0.305
CLEA-0.2-400	65.3 ± 1.9	13.5 ± 0.4	0,12	−0,01	0,19	5.79	0.135
CLEA-0.2-600	42.9 ± 3.5	17.7 ± 1.4	0,38	0,04	0,77	2.79	0.101
CLEA-0.6-200	18.6 ± 3.5	81.5 ± 15.3	0,79	0,006	183166	44.2	0.178
CLEA-0.6-400	103 ± 21	31.4 ± 6.4	0,74	0,025	49,72	0.84	0.094
CLEA-0.6-600	133 ± 29	34.4 ± 7.5	1,04	0,04	7,65	18.7	0.172
CLEA-1.0-200	77.1 ± 0.3	47.6 ± 0.2	19,3	0,34	0,34	20.8	0.159
CLEA-1.0-400	14.7 ± 3.2	68.6 ± 14.9	1,32	0,13	0,125	62.3	0.301
CLEA-1.0-600	24.4 ± 0.2	22.3 ± 0.2	0,76	0,027	41,2	16.5	0.123
CLEA-0.5-1	35.6 ± 4.1	13.6 ± 7.9	0,32	0,03	1,27	1.51	0.379
CLEA-0.5-100	156 ± 6	38.5 ± 4.6	0,76	0,026	56,8	5.84	0.334
CLEA-1.5-1	69 ± 14	82.9 ± 17.3	0,80	0,006	34,8	7.00	0.259
CLEA-1.5-100	39.5 ± 1.9	118.5 ± 1.8	1,58	0,03	0,03	78.48	1.103
CLEA1.0-0.05	21.2 ± 6.6	85.5 ± 28.6	13,4	0,025	0,025	2.77	ND
CLEA-1.0-50.5	78.8 ± 9.9	60.0 ± 4.8	0,51	0,027	15048	7.54	0.565
CLEA-1.0-136	737 ± 24	68.8 ± 2.7	0,465	0,024	2,62	1.29	0.539
CLEA-0.134-50.5	186 ± 10	37.2 ± 3.2	0,413	0,005	0,117	11.9	ND
CLEA-1.867-50.5	16.0 ± 2.9	91.8 ± 2.5	1,58	0,0264	0,0259	81.1	1.094
Free laccase	284 ± 33	21.3 ± 2.5	0,433	0,0189	0,0997	19.7	0.787

*The values represent means of duplicate experiments for the specific activity and thermal stability ± standard deviation.
^aResidual activity after 24 hours at 40°C and pH 3.
ND: Not determined.