Fluorescence Polarization Binding Assay for Aspergillus fumigatus Virulence Factor UDP-Galactopyranose Mutase

Figure 5

FP binding assay to determine 𝐾_𝑑 of the chromophores. (a) Chromophores 1 (○) and 2 (⬤) (excitation at 492 nm and emission at 524 nm). (b) Chromophores 3 (⬤) and 4 (○) (excitation at 544 nm and emission at 584 nm).

(a)

(b)