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Enzyme Research
Volume 2011, Article ID 513905, 9 pages
Research Article

Fluorescence Polarization Binding Assay for Aspergillus fumigatus Virulence Factor UDP-Galactopyranose Mutase

1Department of Biochemistry, Virginia Tech, Blacksburg, VA 26061, USA
2Enzyme Research and Drug Discovery Laboratory, Virginia Tech, Blacksburg, VA 26061, USA
3Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 26061, USA

Received 2 April 2011; Accepted 20 May 2011

Academic Editor: Qi-Zhuang Ye

Copyright © 2011 Jun Qi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The Supporting Material includes the UV-visible spectrum and SDS-PAGE of AfUGM, and 1H NMR and High-resolution MS spectra of the four UDP chromophores. UV-visible spectrum was obtained on Agilent photospectrometer. NMR spectral data were obtained using a JEOL Eclipse spectrometer at 500 MHz, or a Varian Inova spectrometer at 400 MHz. Chemical shifts were reported as σ-values relative to known solvent residue peaks. High-resolution mass spectra (HRMS) were obtained in the Mass Spec Incubator of Department of Biochemistry at Virginia Tech.

  1. Supplementary Material