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Enzyme Research
Volume 2011, Article ID 523420, 8 pages
Review Article

Canonical and Alternative Pathways in Cyclin-Dependent Kinase 1/Cyclin B Inactivation upon M-Phase Exit in Xenopus laevis Cell-Free Extracts

Cell Cycle Group, Institute of Genetics & Development, University of Rennes 1, CNRS-UMR 6061, Faculty of Medicine, 2 Avenue Prof. Léon Bernard, CS 34317, 35043 Rennes Cedex, France

Received 25 February 2011; Revised 1 April 2011; Accepted 18 April 2011

Academic Editor: Heung Chin Cheng

Copyright © 2011 Jacek Z. Kubiak and Mohammed El Dika. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cyclin-Dependent Kinase 1 (CDK1) is the major M-phase kinase known also as the M-phase Promoting Factor or MPF. Studies performed during the last decade have shown many details of how CDK1 is regulated and also how it regulates the cell cycle progression. Xenopus laevis cell-free extracts were widely used to elucidate the details and to obtain a global view of the role of CDK1 in M-phase control. CDK1 inactivation upon M-phase exit is a primordial process leading to the M-phase/interphase transition during the cell cycle. Here we discuss two closely related aspects of CDK1 regulation in Xenopus laevis cell-free extracts: firstly, how CDK1 becomes inactivated and secondly, how other actors, like kinases and phosphatases network and/or specific inhibitors, cooperate with CDK1 inactivation to assure timely exit from the M-phase.