An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD+-Dependent Glutamate Dehydrogenase

Table 1

Sequences of oligonucleotides used in this study.

Name	Sequence 5′-3′	Corresponding position in wildtype gene
A242Gf	GCGCCCCATMCTACGTTACC	921–940
A242Gr	GGTAACGTAGKATGGGGCGC	921–940
F238Sf	GCATTAGCAGGTTCTGGTAACGTAGCATGGGG	906–937
F238Sr	CCCCATGCTACGTTACCAGAACCTGCTAATGC	906–937
P262Sf	GTTACACTTTCCGGATCAGACGGATACATCTACG	974–1007
P262Sr	CGTAGATGTATCCGTCTGATCCGGAAAGTGTAAC	974–1007
D263Kf	CAGTTACACTTTCCGGACCAAAGGGATACATCTACGATCCG	973–1012
D263Kr	CGGATCGTAGATGTATCCCTTTGGTCCGGAAAGTGTAACTG	973–1012
Seq	TCCTATGGGTGGTGCCAAAG	563–582