Study of Soybean Oil Hydrolysis Catalyzed by Thermomyces lanuginosus Lipase and Its Application to Biodiesel Production via Hydroesterification

Figure 6

Reaction kinetics of simple batch and fed-batch hydrolysis of soybean oil by TL 100L lipase. The reaction conditions were 2.3% (v/v) lipase (for the fed-batch this volume of enzyme was divided into 3 equal parts that were added at times zero, 6, and 12 hours), 62% (v/v) soybean oil, 10 mM buffer (pH 8.0), at 60^ºC, for 24 hours.