Research Article

Study of Soybean Oil Hydrolysis Catalyzed by Thermomyces lanuginosus Lipase and Its Application to Biodiesel Production via Hydroesterification

Table 2

CCRD matrix and the results of soybean oil hydrolysis by lipase TL 100L. The reactions were carried out in 10 mM sodium phosphate buffer (pH 8.0) for 19 hours.

AssayTemperature ( )Concentration of lipase (% v/v)Concentration of oil (% v/v)Final concentration of FFAs (% w/w)

1502.36280.4
2602.36285.4
3507.76285.6
4607.76285.6
5502.38466.9
6602.38472.5
7507.78474.3
8607.78471.7
9475.07378.6
10635.07381.0
11550.57370.1
12559.57381.7
13555.05586.8
14555.09166.1
15555.07382.0
16555.07382.1
17555.07384.0
18555.07383.1