Enzyme Mechanism and Slow-Onset Inhibition of Plasmodium falciparum Enoyl-Acyl Carrier Protein Reductase by an Inorganic Complex

Figure 9

Two-step inactivation of PfENR by [Fe^II(CN)₅(INH)]³⁻. The values for the apparent first-order rate constant of inactivation (𝑘_inact) were obtained from a plot of residual enzyme activity versus time (not shown). The 𝑘_inact values were plotted against [Fe^II(CN)₅(INH)]³⁻ concentration in the preincubation mixture. The data were fitted to (6).