Research Article

Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology

Figure 4

ODC activity characterization. (a) Plot of Lineweaver-Burk. ODC activity was assayed in vitro at different concentrations of the substrate ornithine to estimate apparent Km and Vmax. (b) The Y-GFP-ODC clone was cultured in absence or presence of 5 mM α-difluoromethylornithine (DFMO) for 24 hs, then cells were harvested, washed and ODC activity was measured. (c) ODC activity was carried out in the presence of DFMO at different concentrations. Values in B and C are given as percentages of maximal activity. All other details as indicated in Materials and Methods. These values are the average of two independent experiments.
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