Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective 𝜶-L-Rhamnosidase and 𝜷-D-Glucosidase Activities of Naringinase

Table 4

Effects and respective significance levels (P) of temperature (T) and pH on α-L-rhamnosidase residual activity (𝐴_𝑟) and on first deactivation rate coefficient (𝑘₁) for α-L-rhamnosidase and β-D-glucosidase activities of naringinase.

Variable	𝐴𝑟	𝑘1	𝑘1
	(α-L-rhamnosidase)	(α-L-rhamnosidase)	(β-D-glucosidase)
T (linear term)	18.1467***	0.0509***	1.0856***
T (quadratic term)	−23.8667^∗∗∗	0.0188**	0.6455***
pH (linear term)	−2.5064^∗	0.0052	0.1745***
pH (quadratic term)	−2.4592^∗	0.0211**	0.9921***
𝑇×pH	−2.3500	0.0055	0.223***

^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001.