Research Article

Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective -L-Rhamnosidase and -D-Glucosidase Activities of Naringinase

Table 6

Verification of the developed model for β-D-glucosidase inactivation at optimal temperature and pH conditions leading the best α-L-rhamnosidase residual activity of naringinase.

PredictedExperimental

Temperature (°C)81.581.5
pH3.93.9
Time (min)1616
α-L-rhamnosidase residual activity0.770.78
β-D-glucosidase residual activity0.00010.0001