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Enzyme Research
Volume 2011 (2011), Article ID 796407, 6 pages
http://dx.doi.org/10.4061/2011/796407
Research Article

Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes

1Department of Microbiology and Microbial Technology, College of Biotechnology and Allied Sciences, Allahabad Agricultural Institute-Deemed University, Uttar Pradesh, Allahabad-211007, India
2College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi Arabia
3Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
4Department of Biological Sciences, School of Basic Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Uttar Pradesh, Allahabad 211 007, India

Received 19 December 2010; Revised 20 February 2011; Accepted 23 February 2011

Academic Editor: Alane Beatriz Vermelho

Copyright © 2011 Babu Joseph et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Production of cold active lipase by semisolid state fermentation involves the use of agroindustrial residues. In the present study, semisolid state fermentation was carried out for the production of cold active lipase using Micrococcus roseus, isolated from soil samples of Gangotri glaciers, Western Himalayas. Among various substrate tested, groundnut oil cake (GOC) favored maximal yield of lipases at 15 ± 1°C within 48 h. Supplementation of glucose 1% (w/v) as additional carbon source and ammonium nitrate 2% (w/v) as additional nitrogen source enhanced production of lipase. Addition of triglycerides 0.5% (v/v) tends to repress the lipase production. Further mixed preparation of groundnut oil cake (GOC) along with mustard oil cake (MOC) in the ratio of 1 : 1, and its optimization resulted in improved production of cold active lipase. The enzyme exhibited maximum activity at 10–15°C and was stable at temperatures lower than 30°C. The lipase exhibited optimum activity at pH 8 and showed more than 60% stability at pH 9. Semisolid state fermentation process by utilizing agroindustrial wastes will direct to large-scale commercialization of lipase catalyzed process in cost-effective systems.